The ability to activate brown fat (BAT) in response to cold, associates with cardiometabolic health, whereas accumulation of lipids in the visceral adipose depot associates with cardiometabolic disease. The underlying mechanisms for these correlations remains elusive and motivates further comparisons of adipose depots in humans. The prevalence of cold-activated BAT declines with age, whereas dormant BAT exist in most adults. Seasonal and individual differences suggest a plasticity in BAT activity and a potential for BAT reactivation. If BAT play an active role in metabolic regulation, restoring this function could improve human metabolic health. For deeper insight into common and distinct features of human adipose depots, we performed single cell transcriptomics on adipose stem and progenitor cells (APSCs) derived from four different human BAT and white adipose tissue depots. Interestingly, cells from all depots separated into two main cell fates during early differentiation in vitro: a metabolic/adipogenic and a structural/ fibrogenic cell fate. Future studies should address whether a regulation of the separation between fibrogenic and adipogenic cell types is involved in the age related declines in BAT metabolism and the association to cardiometabolic disease.